The Omega project aims to use CRISPR/Cas and the moss Physcomitrium patens to develop a synthetic biology platform to modify plant genomes at the megabase (Mb) scale. The main objectives of this project are to (1) perform a genome-wide CRISPR screen to determine gene essentiality in lab conditions, (2) find an approach to perform precise and efficient Mb deletions of non-coding DNA, and (3) use site-specific recombination for genomic restructuring.